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ng well recombinant sars cov 2 spike protein  (R&D Systems)


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    Structured Review

    R&D Systems ng well recombinant sars cov 2 spike protein
    Ng Well Recombinant Sars Cov 2 Spike Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ng+well+recombinant+sars+cov+2+spike+protein/Recombinant+SARS-CoV-2+Spike+His+Protein%2C+CF/pm41893460-77-12-17
    Average 95 stars, based on 72 article reviews
    ng well recombinant sars cov 2 spike protein - by Bioz Stars, 2026-10
    95/100 stars

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    Recombinant:

    Article Title: SARS-CoV-2 Anti-Spike IgG Subclass Titres in a Population with Prior Exposure to Perfluorooctanoic Acid (PFOA).
    Article Snippet: .. Briefly, high-binding plates (Greiner Bio-One GmbH, Kremsmünster, Austria) were coated with 100 ng/well recombinant SARS-CoV-2 spike protein (R&D Systems, Minneapolis, MN, USA) suspended in coating buffer (8.4 g/L NaHCO3; 3.56 g/L Na2CO3 in H2O; pH = 9.6). ..



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    Construction of recombinant LSDV-SARS2-S,N. ( A ) Schematic diagrams of the wild type and modified <t>SARS-CoV-2</t> spike antigens with amino acid residue positions labeled above. The native signal sequence (SS) was replaced with the tissue plasminogen activator signal sequence (TPA), the furin cleavage site (RRAR) was replaced with a short linker sequence (GSAS), and two stabilizing proline mutations were included (K986P and V987P). ( B ) Schematic diagram of the transfer vector LSDV-Red SARS-CoV2-S+N used for homologous recombination with the parent virus LSDV(SODis)BEFV-Gb genome between ORFs 49 and 50 to generate LSDV-SARS2-S,N. ( C ) Generation and passage of LSDV-SARS2-S,N. The recombinant virus (mCherry; red) was constructed in LT cells at passage 0 (P0) by infection with LSDV(SODis)BEFV-Gb (eGFP; green) and transfection with the transfer vector. P1 = first passage in MDBK cells, P2 = second passage in MDBK cells, P3 = third passage in MDBK cells. All scale bars = 50 µm.
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    Image Search Results


    Construction of recombinant LSDV-SARS2-S,N. ( A ) Schematic diagrams of the wild type and modified SARS-CoV-2 spike antigens with amino acid residue positions labeled above. The native signal sequence (SS) was replaced with the tissue plasminogen activator signal sequence (TPA), the furin cleavage site (RRAR) was replaced with a short linker sequence (GSAS), and two stabilizing proline mutations were included (K986P and V987P). ( B ) Schematic diagram of the transfer vector LSDV-Red SARS-CoV2-S+N used for homologous recombination with the parent virus LSDV(SODis)BEFV-Gb genome between ORFs 49 and 50 to generate LSDV-SARS2-S,N. ( C ) Generation and passage of LSDV-SARS2-S,N. The recombinant virus (mCherry; red) was constructed in LT cells at passage 0 (P0) by infection with LSDV(SODis)BEFV-Gb (eGFP; green) and transfection with the transfer vector. P1 = first passage in MDBK cells, P2 = second passage in MDBK cells, P3 = third passage in MDBK cells. All scale bars = 50 µm.

    Journal: Viruses

    Article Title: LSDV-Vectored SARS-CoV-2 S and N Vaccine Protects against Severe Clinical Disease in Hamsters

    doi: 10.3390/v15071409

    Figure Lengend Snippet: Construction of recombinant LSDV-SARS2-S,N. ( A ) Schematic diagrams of the wild type and modified SARS-CoV-2 spike antigens with amino acid residue positions labeled above. The native signal sequence (SS) was replaced with the tissue plasminogen activator signal sequence (TPA), the furin cleavage site (RRAR) was replaced with a short linker sequence (GSAS), and two stabilizing proline mutations were included (K986P and V987P). ( B ) Schematic diagram of the transfer vector LSDV-Red SARS-CoV2-S+N used for homologous recombination with the parent virus LSDV(SODis)BEFV-Gb genome between ORFs 49 and 50 to generate LSDV-SARS2-S,N. ( C ) Generation and passage of LSDV-SARS2-S,N. The recombinant virus (mCherry; red) was constructed in LT cells at passage 0 (P0) by infection with LSDV(SODis)BEFV-Gb (eGFP; green) and transfection with the transfer vector. P1 = first passage in MDBK cells, P2 = second passage in MDBK cells, P3 = third passage in MDBK cells. All scale bars = 50 µm.

    Article Snippet: ELISA plates were coated overnight with 10 ng/well recombinant E. coli -produced SARS-CoV-2 spike protein (Invitrogen, RP-87668).

    Techniques: Recombinant, Modification, Residue, Labeling, Sequencing, Plasmid Preparation, Homologous Recombination, Virus, Construct, Infection, Transfection

    Immunogenicity of LSDV-SARS2-S,N in mice. ( A ) Schematic of the timing of immunizations and blood draws. ( B ) Frequency of antigen-specific T cells recognizing SARS-CoV-2 peptide pools. (SFU, spot-forming units). ( C ) Quantification of spike-specific antibodies by ELISA over the course of the experiment. The antibody titers are presented for pooled sera at each time point. ( D ) Terminal neutralizing antibody titers against the matched virus from which the vaccines were derived. (ID 50 , half maximal inhibitory dilution).

    Journal: Viruses

    Article Title: LSDV-Vectored SARS-CoV-2 S and N Vaccine Protects against Severe Clinical Disease in Hamsters

    doi: 10.3390/v15071409

    Figure Lengend Snippet: Immunogenicity of LSDV-SARS2-S,N in mice. ( A ) Schematic of the timing of immunizations and blood draws. ( B ) Frequency of antigen-specific T cells recognizing SARS-CoV-2 peptide pools. (SFU, spot-forming units). ( C ) Quantification of spike-specific antibodies by ELISA over the course of the experiment. The antibody titers are presented for pooled sera at each time point. ( D ) Terminal neutralizing antibody titers against the matched virus from which the vaccines were derived. (ID 50 , half maximal inhibitory dilution).

    Article Snippet: ELISA plates were coated overnight with 10 ng/well recombinant E. coli -produced SARS-CoV-2 spike protein (Invitrogen, RP-87668).

    Techniques: Enzyme-linked Immunosorbent Assay, Virus, Vaccines, Derivative Assay

    Vaccine-mediated protection against heterologous challenge. ( A ) Change in weight following experimental challenge with SARS-CoV-2 Delta (10 4 pfu) 2-way ANOVA. * = p < 0.05 ( B ) Grading of lung pathology following challenge (t-test). ( C ) Representative images of histopathology findings in lung sections. Scale bars = 50 µm. ( D ) SARS-CoV-2 RNA viral load in the lungs, following challenge (t-test).

    Journal: Viruses

    Article Title: LSDV-Vectored SARS-CoV-2 S and N Vaccine Protects against Severe Clinical Disease in Hamsters

    doi: 10.3390/v15071409

    Figure Lengend Snippet: Vaccine-mediated protection against heterologous challenge. ( A ) Change in weight following experimental challenge with SARS-CoV-2 Delta (10 4 pfu) 2-way ANOVA. * = p < 0.05 ( B ) Grading of lung pathology following challenge (t-test). ( C ) Representative images of histopathology findings in lung sections. Scale bars = 50 µm. ( D ) SARS-CoV-2 RNA viral load in the lungs, following challenge (t-test).

    Article Snippet: ELISA plates were coated overnight with 10 ng/well recombinant E. coli -produced SARS-CoV-2 spike protein (Invitrogen, RP-87668).

    Techniques: Histopathology